Dr. Bali Pulendran’s lab in the Institute for Immunity, Transplantation and Infection (ITI) is seeking a highly motivated Life Science Research Professional 1 (LSRP 1) to join their team and perform basic functions and activities involved in defined research projects, and independently conduct and analyze experiments.
ITI is an interdisciplinary institute and the overall goal of the lab's research is to understand how the innate immune system regulates adaptive immune responses to pathogens and harness this knowledge in the design of vaccines and therapeutics. Towards this end, we are using an integrative systems biological approach that use high throughput assays (transcriptomics, micro RNA expression, metabolomics) combined with computational approaches.
The LSRP 1 primary duty will be to perform DNA digests, genotyping using PCR, analysis via gel electrophoresis and visualization, compiling results and uploading to an on-line database system for a 70+ strain mouse colony and assist with mouse colony maintenance. The LSRP will also work with research personnel and will use a variety of knockout and transgenic mice to perform research experiments using molecular biology techniques which include PCR, genotyping, cloning, DNA extraction; biochemical assays, animal surgeries, cell culture techniques, histology, immunochemistry in animals. The goal of the work will be to further understand how the immune system works and to help develop the best approach to vaccine development. The LSRP will be expected to troubleshoot existing protocols and develop novel protocols and procedures as well as provide technical and scientific advice to other research personnel in lab.
Secondary duties will include lab maintenance to facilitate the work of the lab. The position will also entail lab support tasks including maintenance of lab records and adhering to safety regulations.
Duties include:
• Plan approach to experiments in support of research projects in lab and/or field based on knowledge of scientific theory. • Independently conduct experiments; maintain detailed records of experiments and outcomes. • Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher. • Tissue digestion and PCR to genotype many mouse strains, analysis of results after gel electrophoresis and visualization, compilation and entry of results in a database • Design primers for genotyping experiments • Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research. • Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals. • Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed. • Assist with orientation and training of new staff or students on lab procedures or techniques.
*- Other duties may also be assigned The job duties listed are typical examples of work performed by positions in this job classification and are not designed to contain or be interpreted as a comprehensive inventory of all duties, tasks, and responsibilities. Specific duties and responsibilities may vary depending on department or program needs without changing the general nature and scope of the job or level of responsibility. Employees may also perform other duties as assigned.
The expected pay range for this position is $26.44 to $36.54 per hour. Stanford University provides pay ranges representing its good faith estimate of what the university reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location and external market pay for comparable jobs.
At Stanford University, base pay represents only one aspect of the comprehensive rewards package. The Cardinal at Work website (https://cardinalatwork.stanford.edu/benefits-rewards) provides detailed information on Stanford’s extensive range of benefits and rewards offered to employees. Specifics about the rewards package for this position may be discussed during the hiring process.
Consistent with its obligations under the law, the University will provide reasonable accommodations to applicants and employees with disabilities. Applicants requiring a reasonable accommodation for any part of the application or hiring process should contact Stanford University Human Resources at stanfordelr@stanford.edu. For all other inquiries, please submit a contact form.
Stanford is an equal employment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law.
DESIRED QUALIFICATIONS: B.S. in Molecular Biology, Genetics or similar field or a related scientific field Experience with PCR and gel electrophoresis Knowledge of basic biology and molecular biology/genetics Skills to coordinate activities between units and investigators, and to consult with investigators regarding their research projects. Some background in mammalian genetics and gene editing techniques. Excellent organizational and planning skills using computerized data bases for scheduling procedures, time management, data entry, graph preparation, etc. Ability to work efficiently within an on-line database system. Some prior experience working with mice. Should be willing and comfortable handling mice
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED): • General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed. • Demonstrated ability to apply theoretical knowledge of science principals to problem solve work. • Ability to maintain detailed records of experiments and outcomes. • General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications. • Ability to work under deadlines with general guidance. • Excellent organizational skills and demonstrated ability to accurately complete detailed work.
CERTIFICATIONS & LICENSES:
None
PHYSICAL REQUIREMENTS*: • Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds. • Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts. • Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls. • Must have correctible vision to perform duties of the job. • Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours. • Ability to lift heavy objects weighing up to 50 pounds. • Ability to work in a dusty, dirty, and odorous environment. • Position may require repetitive motion.
*- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.
WORKING CONDITIONS:
• May require working in close proximity to blood borne pathogens. • May require work in an environment where animals are used for teaching and research. • Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present. • Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator. • May require extended or unusual work hours based on research requirements and business needs.
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